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Comparative secretome analysis after particulate exposure in human lung cell culture models

ABSTRACT: 2D (A549) and 3D (AIR-606) human lung epithelial cell cultures were mock treated or exposed to 100 ug/ml petroleum coke or urban air particulates (both in the PM10 range) for 16 h. Secretomes were compared for triplicate treatments for each group by isobaric tags and LC/MS/MS.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Pulmonary Fibrosis

SUBMITTER: Joseph Caruso

LAB HEAD: Dr. Paul Stemmer

PROVIDER: PXD005194 | Pride | 2018-03-26

REPOSITORIES: Pride

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Petroleum coke exposure leads to altered secretome profiles in human lung models.

Caruso J A JA Stemmer P M PM

Human & experimental toxicology 20180326 11

Petroleum coke (PC) is a coal-like product that is produced during the refinement of crude oil and bituminous sand. Fugitive dust from open storage of PC in urban areas is a potential human health concern. Animal inhalation studies suggest that PC leads to an adverse pulmonary histopathology, including areas of fibrosis and chronic inflammation; however, little is known about its impact on human health. In order to identify biomarkers and cellular pathways that are associated with exposure, we p ...[more]

PMID: 29577758

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