Proteomics

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A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function


ABSTRACT: One key concept in the evolution of new functions is the ability of enzymes to perform promiscuous side-reactions that serve as a source of novelty that may become beneficial under certain conditions. Here, we identify a mechanism where a bacteriophage-encoded enzyme introduces novelty by inducing expression of a promiscuous bacterial enzyme. By screening for bacteriophage DNA that rescued an auxotrophic E. coli mutant carrying a deletion of the ilvA gene, we show that bacteriophage-encoded S-adenosylmethionine (SAM) hydrolases reduce SAM levels. Via this perturbation of bacterial metabolism, expression of the promiscuous bacterial enzyme MetB is increased, which in turn complements the absence of IlvA. These results demonstrate how foreign DNA can increase the metabolic capacity of bacteria, not only by transfer of bona fide new genes, but also by bringing cryptic bacterial functions to light via perturbations of cellular physiology.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Escherichia Coli

SUBMITTER: Egor Vorontsov  

LAB HEAD: Dan I. Andersson

PROVIDER: PXD005236 | Pride | 2018-05-11

REPOSITORIES: Pride

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Publications

A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function.

Jerlström Hultqvist Jon J   Warsi Omar O   Söderholm Annika A   Knopp Michael M   Eckhard Ulrich U   Vorontsov Egor E   Selmer Maria M   Andersson Dan I DI  

Nature ecology & evolution 20180528 8


One key concept in the evolution of new functions is the ability of enzymes to perform promiscuous side-reactions that serve as a source of novelty that may become beneficial under certain conditions. Here, we identify a mechanism where a bacteriophage-encoded enzyme introduces novelty by inducing expression of a promiscuous bacterial enzyme. By screening for bacteriophage DNA that rescued an auxotrophic Escherichia coli mutant carrying a deletion of the ilvA gene, we show that bacteriophage-enc  ...[more]

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