Chlamydomonas reinhardtii Mannosidase 1A and Xylosyltransferase1A N-glycosylation LC/MS-MS

ABSTRACT: We have identified insertional mutants of mannosidase 1A and xylosyltransferase 1A in Chlamydomonas reinhardtii. Mass spectrometric analyses of intact N-glycopeptides revealed that disruption of the genes altered the N-glycan composition of the alga (in single as well as double mutant). In contrast to wildtype, methylated hexoses and terminal xylose were nearly absent in N-glycopeptides from the Man1A mutant strain whereas the XylT1A mutant showed a lack of core xylose and excessively trimmed N-glycans. The double mutant did not show this excessive trimming, thus Man1A-dependent trimming can be concluded in C. reinhardtii. Furthermore, these data indicate that methylation of hexoses is tightly interlinked with Man1A function, pointing to an enzymatic cascade in the N-glycosylation pathway.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Culture Supernatant, Photosynthetic Cell

SUBMITTER: Stefan Schulze  

LAB HEAD: Michael Hippler

PROVIDER: PXD005254 | Pride | 2018-02-27

REPOSITORIES: Pride