Proteomics

Dataset Information

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Chlamydomonas reinhardtii quantification of Mannosidase 1A and Xylosyltransferase1A

ABSTRACT: We have identified insertional mutants of mannosidase 1A and xylosyltransferase 1A in Chlamydomonas reinhardtii. To verify a knockout/knockdown of the enzymes in the insertional mutant strains, PRM measurements were performed. Mass spectrometric analyses of intact N-glycopeptides revealed that disruption of the genes altered the N-glycan composition of the alga (in single as well as double mutant).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Plant Cell, Cell Culture

SUBMITTER: Stefan Schulze  

LAB HEAD: Michael Hippler

PROVIDER: PXD005257 | Pride | 2018-02-27

REPOSITORIES: Pride

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Publications

<i>N</i>-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of <i>Chlamydomonas</i><i>reinhardtii</i>.

Schulze Stefan S   Oltmanns Anne A   Machnik Nick N   Liu Gai G   Xu Nannan N   Jarmatz Niklas N   Scholz Martin M   Sugimoto Kazuhiko K   Fufezan Christian C   Huang Kaiyao K   Hippler Michael M  

Plant physiology 20171229 3

At present, only little is known about the enzymatic machinery required for <i>N</i>-glycosylation in <i>Chlamydomonas reinhardtii</i>, leading to the formation of <i>N</i>-glycans harboring Xyl and methylated Man. This machinery possesses new enzymatic features, as <i>C. reinhardtii N</i>-glycans are independent of β1,2-<i>N</i>-acetylglucosaminyltransferase I. Here we have performed comparative <i>N</i>-glycoproteomic analyses of insertional mutants of mannosidase 1A (IM <i><sub>Man1A</sub></i  ...[more]

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