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Proteomic survey for search of biomarkers of colon tumors

ABSTRACT: To the search of new colon tumor biomarkers in the transition from normal colon (NC) mucosa to adenoma (AD) and adenocarcinoma (AC), we integrated microarray data with the results of a high-throughput proteomic workflow. In proteomic study, we used a modified isoelectric focusing protocol on strips with an immobilized pH gradient to separate peptides labeled with iTRAQ (isobaric tags for relative and absolute quantitation) tags followed by liquid chromatography–tandem mass spectrometry analysis.

INSTRUMENT(S): Q-Tof Premier

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Colon

DISEASE(S): Colon Cancer

SUBMITTER: Michał Kistowski

LAB HEAD: Michał Dadlez

PROVIDER: PXD005274 | Pride | 2016-12-14

REPOSITORIES: Pride

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Integrating proteomic and transcriptomic high-throughput surveys for search of new biomarkers of colon tumors.

Mikula Michal M Rubel Tymon T Karczmarski Jakub J Goryca Krzysztof K Dadlez Michal M Ostrowski Jerzy J

Functional & integrative genomics 20101109 2

To the search of new colon tumor biomarkers in the transition from normal colon (NC) mucosa to adenoma (AD) and adenocarcinoma (AC), we integrated microarray data with the results of a high-throughput proteomic workflow. In proteomic study, we used a modified isoelectric focusing protocol on strips with an immobilized pH gradient to separate peptides labeled with iTRAQ (isobaric tags for relative and absolute quantitation) tags followed by liquid chromatography-tandem mass spectrometry analysis. ...[more]

PMID: 21061036

Publication: 1/2

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