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The diurnal liver acetylome is regulated by circadian and feeding rhythms-orchestrated deacetylation - total mouse liver extract

ABSTRACT: Acetylation of lysine is involved in various biological processes and is considered as a key reversible post-translational modification (PTM) in the regulation of gene expression, enzyme activity, protein-protein interaction, and subcellular localization. This PTM is therefore highly relevant in the context of circadian biology but its characterization at the genome wide scale and its clock-dependent or -independent regulation is still poorly characterized. Here we used in vivo stable isotope labeling by amino acids (SILAC) coupled with an enrichment of acetylated-lysine-containing peptides to provide a comprehensive and rhythmic acetylome map of the liver.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Loïc Dayon

LAB HEAD: Frédéric Gachon

PROVIDER: PXD005317 | Pride | 2017-08-02

REPOSITORIES: Pride

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Circadian and Feeding Rhythms Orchestrate the Diurnal Liver Acetylome.

Mauvoisin Daniel D Atger Florian F Dayon Loïc L Núñez Galindo Antonio A Wang Jingkui J Martin Eva E Da Silva Laetitia L Montoliu Ivan I Collino Sebastiano S Martin Francois-Pierre FP Ratajczak Joanna J Cantó Carles C Kussmann Martin M Naef Felix F Gachon Frédéric F

Cell reports 20170801 7

Lysine acetylation is involved in various biological processes and is considered a key reversible post-translational modification in the regulation of gene expression, enzyme activity, and subcellular localization. This post-translational modification is therefore highly relevant in the context of circadian biology, but its characterization on the proteome-wide scale and its circadian clock dependence are still poorly described. Here, we provide a comprehensive and rhythmic acetylome map of the ...[more]

PMID: 28813682

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