Increased Hemodynamic Load in Early Embryonic Stages Alters Endocardial to Mesenchymal Transition

ABSTRACT: Normal blood flow is essential for proper heart formation during embryonic development, as abnormal hemodynamic load (blood pressure and shear stress) results in cardiac defects seen in congenital heart disease. However, the progressive detrimental remodeling processes that relate altered blood flow to cardiac defects remain unclear. Outflow tract banding was used to increase hemodynamic load in the chicken embryo heart between Hamburger and Hamilton stages 18 and 24. Increased hemodynamic load induced increased cell density in outflow tract cushions, fewer cells along the endocardial lining, endocardium junction disruption, and altered periostin expression as measured by confocal microscopy analysis. Proteomic mass-spectrometry analysis quantified altered protein composition after banding.

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Heart

DISEASE(S): Congenital Heart Disease

SUBMITTER: Sandra Rugonyi  

LAB HEAD: Sandra Rugonyi

PROVIDER: PXD005362 | Pride | 2018-10-26

REPOSITORIES: Pride