Proteomics

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N-Linked Glycoprotein Profiles of Human Breast Luminal Malignant Cell Lines

ABSTRACT: Secreted proteins and transmembrane proteins with extracellular domains are frequently glycosylated; this group of proteins includes those that participate in the various intercellular junctions and signaling pathways of an epithelium. In this study we characterized the differences in glycoprotein expression between claudin-low and other breast cell lines using a dataset of 26 breast cell lines in which the glycoproteins were identified and quantitated by liquid chromatography/ tandem mass spectrometry. Our goals are to characterize the glycoproteome of a set of claudin-low lines, compare them to basal, luminal and non-malignant cells and to identify drugs that may be especially effective on these cell lines. These seven luminal malignant breast cells (BT474, HCC1428, MCF7, SKBR3, SUM185PE, T47D and ZR751) data are a part of 26 breast cell lines we analyzed.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Ten-Yang Yen  

LAB HEAD: Ten-Yang Yen

PROVIDER: PXD005390 | Pride | 2017-03-22

REPOSITORIES: Pride

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Publications

Glycoproteins in Claudin-Low Breast Cancer Cell Lines Have a Unique Expression Profile.

Yen Ten-Yang TY   Bowen Spencer S   Yen Roger R   Piryatinska Alexandra A   Macher Bruce A BA   Timpe Leslie C LC  

Journal of proteome research 20170321 4

Claudin proteins are components of epithelial tight junctions; a subtype of breast cancer has been defined by the reduced expression of mRNA for claudins and other genes. Here, we characterize the expression of glycoproteins in breast cell lines for the claudin-low subtype using liquid chromatography/tandem mass spectrometry. Unsupervised clustering techniques reveal a group of claudin-low cell lines that is distinct from nonmalignant, basal, and luminal lines. The claudin-low cell lines express  ...[more]

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