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The proteome of Fibrobacter succinogenes S85 outer membrane vesicles

ABSTRACT: Here, we illustrate the proteomic composition and hydrolytic capacity of outer membrane vesicles (OMVs) produced by F. succinogenes during growth on Avicel cellulose and show that the OMVs are equipped with a highly versatile polysaccharide-degrading machinery. Proteomic assessment identified an enrichment of acidic proteins as well as carbohydrate-active enzymes of varying putative functions. Further, we show that the OMVs contain a membrane-bound multiprotein complex containing four proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Fibrobacter Succinogenes Subsp. Succinogenes S85

SUBMITTER: Magnus Arntzen

LAB HEAD: Vincent Eijsink

PROVIDER: PXD005442 | Pride | 2017-06-30

REPOSITORIES: Pride

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Outer membrane vesicles from Fibrobacter succinogenes S85 contain an array of carbohydrate-active enzymes with versatile polysaccharide-degrading capacity.

Arntzen Magnus Ø MØ Várnai Anikó A Mackie Roderick I RI Eijsink Vincent G H VGH Pope Phillip B PB

Environmental microbiology 20170529 7

Fibrobacter succinogenes is an anaerobic bacterium naturally colonising the rumen and cecum of herbivores where it utilizes an enigmatic mechanism to deconstruct cellulose into cellobiose and glucose, which serve as carbon sources for growth. Here, we illustrate that outer membrane vesicles (OMVs) released by F. succinogenes are enriched with carbohydrate-active enzymes and that intact OMVs were able to depolymerize a broad range of linear and branched hemicelluloses and pectin, despite the inab ...[more]

PMID: 28447389

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