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Quantitative proteomic profiling of fibroblasts in oesophageal adenocarcinoma

ABSTRACT: The accumulatiob of cancer-associated fibroblasts in tumours correlates with poor prognosis across cancer types including oesophageal adenocarcinoma. In this study we examined the proteomic profile of cancer-associated fibroblasts compared to normal fibroblasts.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Antigoni Manousopoulou

LAB HEAD: Spiros D. Garbis

PROVIDER: PXD005444 | Pride | 2018-08-13

REPOSITORIES: Pride

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Quantitative proteomic profiling of primary cancer-associated fibroblasts in oesophageal adenocarcinoma.

Manousopoulou Antigoni A Hayden Annette A Mellone Massimiliano M Garay-Baquero Diana J DJ White Cory H CH Noble Fergus F Lopez Monette M Thomas Gareth J GJ Underwood Timothy J TJ Garbis Spiros D SD

British journal of cancer 20180329 9

<h4>Background</h4>Cancer-associated fibroblasts (CAFs) form the major stromal component of the tumour microenvironment (TME). The present study aimed to examine the proteomic profiles of CAFs vs. normal fibroblasts (NOFs) from patients with oesophageal adenocarcinoma to gain insight into their pro-oncogenic phenotype.<h4>Methods</h4>CAFs/NOFs from four patients were sub-cultured and analysed using quantitative proteomics. Differentially expressed proteins (DEPs) were subjected to bioinformatics ...[more]

PMID: 29593339

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