CEGS Proteomics - A multiregional proteomic survey of the postnatal human brain

ABSTRACT: Here we exploit label-free quantitative tandem mass-spectrometry proteomics to create the first in-depth quantitative proteomic survey of regions of the postnatal human brain. We adopted a dual approach to analysing these human brain samples similar to that of a recent high-quality study of the mouse brain proteome by Sharma et al, 2015. First was the discovery phase, designed to create a highly sensitive, heavily fractionated spectral library for each adult brain region. Then, to produce a quantitative run for each adult and postnatal development sample, single shot LC-MS/MS runs were used to accurately quantify proteins based on detected precursor peptide ion mass.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: TuKiet Lam  

LAB HEAD: TuKiet T. Lam

PROVIDER: PXD005445 | Pride | 2018-01-09

REPOSITORIES: Pride