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CEGS Proteomics - A multiregional proteomic survey of the postnatal human brain

ABSTRACT: Here we exploit label-free quantitative tandem mass-spectrometry proteomics to create the first in-depth quantitative proteomic survey of regions of the postnatal human brain. We adopted a dual approach to analysing these human brain samples similar to that of a recent high-quality study of the mouse brain proteome by Sharma et al, 2015. First was the discovery phase, designed to create a highly sensitive, heavily fractionated spectral library for each adult brain region. Then, to produce a quantitative run for each adult and postnatal development sample, single shot LC-MS/MS runs were used to accurately quantify proteins based on detected precursor peptide ion mass.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: TuKiet Lam

LAB HEAD: TuKiet T. Lam

PROVIDER: PXD005445 | Pride | 2018-01-09

REPOSITORIES: Pride

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A multiregional proteomic survey of the postnatal human brain.

Carlyle Becky C BC Kitchen Robert R RR Kanyo Jean E JE Voss Edward Z EZ Pletikos Mihovil M Sousa André M M AMM Lam TuKiet T TT Gerstein Mark B MB Sestan Nenad N Nairn Angus C AC

Nature neuroscience 20171113 12

Detailed observations of transcriptional, translational and post-translational events in the human brain are essential to improving our understanding of its development, function and vulnerability to disease. Here, we exploited label-free quantitative tandem mass-spectrometry to create an in-depth proteomic survey of regions of the postnatal human brain, ranging in age from early infancy to adulthood. Integration of protein data with existing matched whole-transcriptome sequencing (RNA-seq) from ...[more]

PMID: 29184206

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