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Comprehensive proteomic analysis of Penicillium verrucosum

ABSTRACT: In the present study we used OTA producing Penicillium verrucosum to identify and quantify proteins of an organism with yet no protein information available. We were able to identify 3632 proteins in an "ab initio" translated database from DNA sequences of P. verrucosum. Additionally a SWATH analysis was done to find differentially regulated proteins at two different time points of the growth curve. We compared the proteins at the beginning and the end of the log phase.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Penicillium Verrucosum

TISSUE(S): Mycelium

SUBMITTER: Katharina Nöbauer

LAB HEAD: Ebrahim Razzazi-Fazeli

PROVIDER: PXD005459 | Pride | 2017-03-16

REPOSITORIES: Pride

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Comprehensive proteomic analysis of Penicillium verrucosum.

Nöbauer Katharina K Hummel Karin K Mayrhofer Corina C Ahrens Maike M Setyabudi Francis M C FMC Schmidt-Heydt Markus M Eisenacher Martin M Razzazi-Fazeli Ebrahim E

Proteomics 20170501 9

Mass spectrometric identification of proteins in species lacking validated sequence information is a major problem in veterinary science. In the present study, we used ochratoxin A producing Penicillium verrucosum to identify and quantitatively analyze proteins of an organism with yet no protein information available. The work presented here aimed to provide a comprehensive protein identification of P. verrucosum using shotgun proteomics. We were able to identify 3631 proteins in an "ab initio" ...[more]

PMID: 28267294

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