Proteomics

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Analysis of ECM-enriched samples using different methods


ABSTRACT: Using LC-MS/MS, we analyzed ECM-enriched samples obtained from 1) human triple-negative breast cancer samples and matched adjacent normal mammary gland tissues, and 2) disease-free omentum from patient with non-metastatic ovarian cancer and high-grade- serous-ovarian- cancer-derived omental metastasis samples. We conducted the LC-MS/MS analysis on peptides obtained after solubilizing ECM-enriched samples using different methods (crude ECM extract, urea-soluble extract, urea-insoluble extract) and submitted to basic-reversed- phase separation or not.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mammary Gland, Epithelial Ovarian Cancer Cell

DISEASE(S): Ovarian Cancer,Breast Cancer

SUBMITTER: Amanda Del Rosario  

LAB HEAD: Amanda Del Rosario

PROVIDER: PXD005554 | Pride | 2017-07-11

REPOSITORIES: Pride

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