Dataset Information

Mouse retina LC-MSMS - Diurnal expression of proteins in the retina of the blind cone-rod homeobox (Crx(-/-) ) mouse and the 129/Sv mouse: a proteomic study

ABSTRACT: To investigate the role of the circadian clock of the photoreceptor cells in regulation of retinal protein rhythms, we have analyzed diurnal protein expression in the photoreceptor-deficient cone-rod homeobox knock out mouse (Crx-/-). Retinal homogenates of 129/sv and Crx-/- mice were analysed by 2D-PAGE. Differentially expressed spots were in-gel digested with trypsin and identified by LC-MS/MS. Data were used to search the Swiss-Prot protein database.

INSTRUMENT(S): Q-Tof Premier

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Retina

SUBMITTER: Bent Honoré

LAB HEAD: Bent Honoré

PROVIDER: PXD005556 | Pride | 2017-04-04

REPOSITORIES: Pride

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Publications

Diurnal expression of proteins in the retina of the blind cone-rod homeobox (Crx<sup>-/-</sup> ) mouse and the 129/Sv mouse: a proteomic study.

Møller Morten M Rath Martin Fredensborg MF Ludvigsen Maja M Honoré Bent B Vorum Henrik H

Acta ophthalmologica 20170328 7

<h4>Purpose</h4>The vertebrate retina contains a circadian clock participating in adaptations to day and night vision. This peripheral clock is independent of the master clock in the suprachiasmatic nucleus (SCN). The retinal clock is located in several cell types, including the photoreceptors. To investigate the role of the circadian clock of the photoreceptor cells in regulation of retinal protein rhythms, we analysed diurnal protein expression in the photoreceptor-deficient cone-rod homeobox ...[more]

PMID: 28371363

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Project description:NeuroD1 encodes a basic helix-loop-helix transcription factor involved in the development of neural and endocrine structures. NeuroD1 mRNA is highly abundant in the adult mammalian pineal gland and exhibits a developmental expression pattern similar to the retina. This is consistent with the common evolutionary origin of pinealocytes and retinal photoreceptors. Pinealocytes and retinal photoreceptors express a shared set of phototransduction genes and submammalian pinealocytes are photosensitive. In contrast to the retina, the pineal gland is a relatively homogeneous structure, composed 95% of pinealocytes. This makes the pineal gland a particularly useful model for understanding photoreceptor cell biology. The loss of NeuroD1 in the retina results in progressive photoreceptor degeneration and the molecular mechanisms underlying this retinal degeneration phenotype remain unknown. Similarly, the role that NeuroD1 plays in the pineal gland is unknown. To determine the function of NeuroD1 in the pineal gland and retina, a Cre/loxP recombination strategy was used to selectively target a NeuroD1 floxed allele and generate NeuroD1 conditional knockout (cKO) mice. Tissue specificity was conferred using Cre recombinase expressed under the control of the promoter of Crx, a transcription factor selectively expressed in the pineal gland and retina. Pineal and retinal tissues from two-month-old NeuroD1 cKO and control animals were used in microarray studies to identify candidate genes responsible for the photoreceptor degeneration phenotype. The Cre/loxP recombination strategy was used to target a NeuroD1 floxed allele and generate NeuroD1 conditional knockout mice (NeuroD1floxed::Crx-Cre+/-). NeuroD1 floxed mice (NeuroD1floxed::Crx-Cre-/-) served as the controls. Pineal glands and retinas from two-month-old control and conditional knockout mice were collected at ZT6 and ZT20. 3 pools of 6 pineal glands per genotype and respective time of day were collected for each sample. Similarly, 3 pools of 6 retinas each were also collected. RNA from each pool was extracted and hybridized on the Affymetrix Mouse 430 2.0 array.

Dataset Information

Mouse retina LC-MSMS - Diurnal expression of proteins in the retina of the blind cone-rod homeobox (Crx(-/-) ) mouse and the 129/Sv mouse: a proteomic study

Publications

Diurnal expression of proteins in the retina of the blind cone-rod homeobox (Crx<sup>-/-</sup> ) mouse and the 129/Sv mouse: a proteomic study.

Similar Datasets

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