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Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane

ABSTRACT: In the present work neutrophil subcellular fractionation by density gradient centrifugation was conducted in order to perform a quantitative proteomic profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neutrophil, Blood

SUBMITTER: Ole Østergaard

LAB HEAD: Niels Borregaard

PROVIDER: PXD005559 | Pride | 2017-06-23

REPOSITORIES: Pride

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Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane: correlation with transcriptome profiling of neutrophil precursors.

Rørvig Sara S Østergaard Ole O Heegaard Niels H H NH Borregaard Niels N

Journal of leukocyte biology 20130506 4

Neutrophils are indispensable in the innate immune defense against invading microorganisms. Neutrophils contain SVs and several subsets of granules that are essential for their function. Proteins present in neutrophil SVs and granules are synthesized during terminal granulopoiesis in the bone marrow. The heterogeneity of granules, as determined by marker proteins characteristic of each granule subset, is thought to result from differences in the biosynthetic windows of major classes of granule p ...[more]

PMID: 23650620

Publication: 1/2

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