Browse
Submit Data
Databases
API
Help

Dataset Information

363 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metaproteomics on preterm infant stool

ABSTRACT: Development of the gut microbiota is greatly impacted in preterm infants. Despite increasing knowledge about microbiota composition in preterm infants, knowledge about the functional signatures of the intestinal microbiota remains limited. The aim was to study transitions in microbiota activity during the first six postnatal weeks in ten preterm infants. A total of 64 stool samples were measured by LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human) Bacteria

TISSUE(S): Feces

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD005574 | Pride | 2017-07-14

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Metaproteomics reveals functional differences in intestinal microbiota development of preterm infants.

Zwittink Romy D RD van Zoeren-Grobben Diny D Martin Rocio R van Lingen Richard A RA Groot Jebbink Liesbeth J LJ Boeren Sjef S Renes Ingrid B IB van Elburg Ruurd M RM Belzer Clara C Knol Jan J

Molecular & cellular proteomics : MCP 20170706 9

<h4>Objective</h4>Development of the gastrointestinal tract and immune system can be modulated by the gut microbiota. Establishment of the intestinal microbiota, in its turn, is affected by host and environmental factors. As such, development of the gut microbiota is greatly impacted in preterm infants, who have an immature gut and are exposed to factors like hospitalization, caesarean section, antibiotics, and respiratory support.<h4>Design</h4>We analyzed fecal microbiota composition and activ ...[more]

PMID: 28684633

Similar Datasets

Metaproteomics on preterm infant stool

Project description:Development of the gut microbiota is greatly impacted in preterm infants. Despite increasing knowledge about microbiota composition in preterm infants, knowledge about the functional signatures of the intestinal microbiota remains limited. The aim was to study transitions in microbiota activity during the first six postnatal weeks in ten preterm infants. A total of 64 stool samples were measured by LC-MS/MS.

2017-10-17 | MSV000081616 | MassIVE

Preterm infant stool metagenomes

Project description:Longitudinal stool metagenomics from preterm infants within the first month after birth

| PRJEB52938 | ENA

Infant stool

Project description:Preterm infant stool metagenome

| PRJNA721684 | ENA

Metaproteome of preterm infant gut microbiome

Project description:91 preterm infant gut metaproteomes measured in technical duplicate using an eleven salt pulse 2D-LC-MS/MS method. Samples represent 17 preterm infants over the first several weeks of life, of which 6 preterm infants eventually developed necrotizing enterocolitis.

| MSV000086096 | MassIVE

Monocyte signature as a predictor of chronic lung disease in the preterm infant

Project description:The early changes in innate immunity associated with BPD (bronchopulmonary dysplasia) development are incompletely understood. Transcriptome analyses from cord blood of preterm infants indicate a monocyte-centered, profibrotic signature at birth confirming TNFalpha as a critical upstream regulator for the development of BPD.

2023-02-22 | GSE225881 | GEO

Microbiota-associated intestinal methylation changes confer immune-metabolic gene network of NEC in preterm pig model

Project description:Intestinal microbiota colonization is important for intestinal development and health of preterm infants, especially those with extremely low birth weight. Recent studies indicated for a dynamic crosstalk between that gut microbiota and DNA methylome of host intestinal cells. Thereby, we sought to determine the epigenomic and metagenomic consequences of suppression of microbiota colonization in the intestine of preterm neonates to gain insights into biological pathways that shape the interface between the gut microbiota and the preterm intestinal cells. We examined 14 preterm piglets by comparing the conventional preterm neonates with those ones treated with oral antibiotics for genome wide DNA methylation and 16S rDNA microbiome. Our results demonstrated an extensive genome-wide DNA methylation changes in response to the suppression of intestinal microbe colonization, especially genes involved in neonatal immune response signaling and glycol-metabolism pathways were identified. Our study highlights several key genes that might predispose preterm neonates to NEC risk due to their key roles involved in the immune-metabolic networks. Our study not only provided rich omic-data to interpret molecular program in relation with microbiota-associated methylome-proteome network changes, but also confer clinical usage of key gene markers for potential early diagnostics of NEC of preterm neonates.

2017-10-12 | GSE88697 | GEO

Fecal filtrate transfer protects against necrotizing enterocolitis in preterm pigs

Project description:Necrotizing enterocolitis (NEC) is an acute and life-threatening gastrointestinal disorder afflicting preterm infants, which is currently unpreventable. Fecal microbiota transplantation (FMT) is a promising preventative therapy, but potential bacterial infection raise concern. Removal of bacteria from donor feces may reduce this risk while maintaining the NEC-preventive effects. We aimed to assess preclinical efficacy and safety of bacteria-free fecal filtrate transfer (FFT). Using fecal material from healthy suckling piglets, we administered FMT rectally, or cognate FFT either rectally or oro-gastrically to formula-fed preterm, cesarean-delivered piglets as a model for preterm infants, We compared gut pathology and related safety parameters with saline controls, and analyzed ileal mucosal transcriptome to gauge the host e response to FMT and FFT treatments relative to control. Results showed that oro-gastric FFT prevented NEC, whereas FMT did not perform better than control. Moreover, FFT but not FMT reduced intestinal permeability, whereas FMT animals had reduced body weight increase and intestinal growth. Global gene expression of host mucosa responded to FMT but not FFT with increased and decreased bacterial and viral defense mechanisms, respectively. In conclusion, as preterm infants are extremely vulnerable to enteric bacterial infections, rational NEC-preventive strategies need incontestable safety profiles. Here we show in a clinically relevant animal model that FFT, as opposed to FMT, efficiently prevents NEC without any recognizable side effects. If translatable to preterm infants, this could lead to a change of practice and in turn a reduction in NEC burden.

2021-10-01 | GSE158878 | GEO

Metagenomic samples obtained from human infant stool samples

Project description:Impact of lactoferrin supplementation on microbiota development in preterm infants receiving probiotics

| PRJNA531325 | ENA

Preterm infant intestinal microbiota

Project description:Development of the intestinal microbiota in preterm infants during the first six postnatal weeks determined by 454 pyrosequencing of the 16S rRNA gene.

| PRJEB18915 | ENA

Data in support of plasma proteomics study forearly markers of antibiotics dependent necrotising enterocolitis in preterm pigs

Project description:Most hospitalized preterm infants receive antibiotics (AB) in the first days of life to treat or prevent systemic infections. Short-term, early AB treatment may also prevent against the microbiota-dependent serious gut disorder, necrotising enterocolitis (NEC). However, it remains a challenge to predict or early detection of NEC in the first weeks of life and few diagnostic markers exist. Using preterm piglets as models for infants, we hypothesised that proteomic profiling could be used to identify new early plasma biomarkers of NEC with or without prior AB treatment. Preterm newborn pigs were treated with saline (CON) or antibiotics (ampicillin, gentamicin, and metronidazole), given enterally (ENT) or parenterally (PAR), and fed formula for four days to induce NEC. The gut was collected for scoring of NEC lesions and blood was collected for haematology and plasma proteomics

2021-09-08 | PXD015938 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data