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GFPtrap enrichment of CYP83A1 and CYP83B1

ABSTRACT: CYP83A1 and CYP83B1 are important enzymes in the biosynthesis of glucosinolates. However, only few of their interaction partners are known so far. We therefore performed a GFPtrap affinity enrichment using protein extracts from lines expressing tagged CYP83A1 or CYP83B1, or from wild type plants as background control.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seedling

SUBMITTER: Katja Baerenfaller

LAB HEAD: Katja Baerenfaller

PROVIDER: PXD005585 | Pride | 2017-11-30

REPOSITORIES: Pride

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Unravelling Protein-Protein Interaction Networks Linked to Aliphatic and Indole Glucosinolate Biosynthetic Pathways in Arabidopsis.

Nintemann Sebastian J SJ Vik Daniel D Svozil Julia J Bak Michael M Baerenfaller Katja K Burow Meike M Halkier Barbara A BA

Frontiers in plant science 20171129

Within the cell, biosynthetic pathways are embedded in protein-protein interaction networks. In Arabidopsis, the biosynthetic pathways of aliphatic and indole glucosinolate defense compounds are well-characterized. However, little is known about the spatial orchestration of these enzymes and their interplay with the cellular environment. To address these aspects, we applied two complementary, untargeted approaches-split-ubiquitin yeast 2-hybrid and co-immunoprecipitation screens-to identify prot ...[more]

PMID: 29238354

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