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Protease activities induced in the apoplast of the resistant tomato cultivar Hawaii 7996 in response to Ralstonia solanacearum infection

ABSTRACT: In the work presented here we aimed to identify the active proteases secreted in the apoplast of the resistant tomato Hawaii 7996 when challenged with R. solanacearum and compared it with that of the susceptible cultivar Marmande. Using ABPP we characterized a variety-specific induction of papain-like cysteine proteases and serine hydrolases.. Altogether this work denotes the importance of novel proteomic approaches –such as ABPP– to provide new insights on old yet elusive questions regarding the molecular basis of resistance to R. solanacearum.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Ralstonia Solanacearum (strain Gmi1000) (pseudomonas Solanacearum) Solanum Lycopersicum

TISSUE(S): Leaf

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD005603 | Pride | 2018-05-18

REPOSITORIES: Pride

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Protease Activities Triggered by <i>Ralstonia solanacearum</i> Infection in Susceptible and Tolerant Tomato Lines.

Planas-Marquès Marc M Bernardo-Faura Martí M Paulus Judith J Kaschani Farnusch F Kaiser Markus M Valls Marc M van der Hoorn Renier A L RAL Coll Núria S NS

Molecular & cellular proteomics : MCP 20180309 6

Activity-based protein profiling (ABPP) is a powerful proteomic technique to display protein activities in a proteome. It is based on the use of small molecular probes that react with the active site of proteins in an activity-dependent manner. We used ABPP to dissect the protein activity changes that occur in the intercellular spaces of tolerant (Hawaii 7996) and susceptible (Marmande) tomato plants in response to <i>R. solanacearum</i>, the causing agent of bacterial wilt, one of the most dest ...[more]

PMID: 29523767

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