Proteomics

Dataset Information

55

Proteomic analysis of cell cycle arrest and differentiation induction caused by ATPR, a derivative of all-trans retinoic acid, in human gastric cancer SGC-7901 cells


ABSTRACT: Experimental design: Peptides digested from the total cellular proteins were analyzed by reverse phase LC–MS/MS followed by a label-free quantification analysis. The SEQUEST search engine was used to identify proteins and bioinformatics resources were used to investigate the involved pathways for the differentially expressed proteins. Results: 13 down-regulated proteins were identified in the ATPR-treated group. Bioinformatics analysis showed that the effects of ATPR on 14-3-3 might potentially involve the PI3K-AKT-FOXO pathway and P27Kip1 expression. Western blot and RT-PCR analysis showed that ATPR could inhibit AKT phosphorylation, up-regulate the expression of FOXO1A and P27Kip1 at both the protein and mRNA levels, and down-regulate the cytoplasmic expression of cyclin E and CDK2. ATPR-induced G0/G1 phase arrest and differentiation can be ablated if the P27kip1 gene is silenced with sequence-specific siRNA. Conclusions and clinical relevance: ATPR might cause cell cycle arrest and differentiation in SGC-7901 cells by simultaneously inhibiting the phosphorylation of AKT and down-regulating 14-3-3. This change would then enhance the inhibition of cyclin E/CDK2 by up-regulating FOXO1A and P27Kip1. Our findings could be of value for finding new drug targets and for developing more effective differentiation inducer.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo sapiens  

TISSUE(S): Cell Culture

DISEASE(S): Gastric Adenocarcinoma

SUBMITTER: Xia Quan  

LAB HEAD: Quan Xia

PROVIDER: PXD005605 | Pride | 2017-02-13

REPOSITORIES: Pride

altmetric image

Publications

Proteomic analysis of cell cycle arrest and differentiation induction caused by ATPR, a derivative of all-trans retinoic acid, in human gastric cancer SGC-7901 cells.

Xia Quan Q   Zhao Yingli Y   Wang Jiali J   Qiao Wenhao W   Zhang Dongling D   Yin Hao H   Xu Dujuan D   Chen Feihu F  

Proteomics. Clinical applications 20170306 7-8


4-amino-2-trifluoromethyl-phenyl retinate (ATPR) was reported to potentially inhibit proliferation and induce differentiation activity in some tumor cells. In this study, a proteomics approach was used to investigate the possible mechanism by screening the differentially expressed protein profiles of SGC-7901 cells before and after ATPR-treatment in vitro.Peptides digested from the total cellular proteins were analyzed by reverse phase LC-MS/MS followed by a label-free quantification analysis. T  ...[more]

Similar Datasets

2014-01-08 | E-GEOD-53855 | ArrayExpress
2013-07-01 | E-GEOD-37409 | ArrayExpress
2017-07-01 | E-MTAB-5649 | ArrayExpress
2015-05-12 | E-GEOD-59727 | ArrayExpress
2019-04-23 | PXD013038 | Pride
2016-07-01 | E-GEOD-81889 | ArrayExpress
2019-02-18 | PXD011095 | Pride
2015-09-02 | E-GEOD-58093 | ArrayExpress
2014-05-19 | E-GEOD-16480 | ArrayExpress
2015-12-09 | E-GEOD-71898 | ArrayExpress