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Ave1 Homologs in Venturia pirina and Venturia inequalis

ABSTRACT: This dataset relates to a proteomic analysis of Ave1 homologs in the fungal pathogens Venturia pirina and Venturia inequalis. These species are the cause of scab disease on European pear (Pyrus communis) and apple (Malus pumila). Sample preparation for both V. pirina and V. inequalis was designed to focus on effectors and eliminate host proteins. To do this the fungi were grown in vitro, on cellophane sheets mimicing the growth habit in infected leaves and proteins were extracted using a gentle washing procedure designed to avoid cell wall breakage. LC-MS/MS data was queried against a protein database generated from gene predictions obtained from whole genome sequences of V. pirina and V. inequalis.

INSTRUMENT(S): LTQ Orbitrap, Bruker Daltonics HCT Series, ultraflex

ORGANISM(S): Venturia Pyrina Venturia Inaequalis

SUBMITTER: Ira Cooke

LAB HEAD: Ira R Cooke

PROVIDER: PXD005614 | Pride | 2022-03-02

REPOSITORIES: Pride

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Project description:Background: Recent studies have identified thousands of sense-antisense gene pairs across different genomes by computational mapping of cDNA sequences. These studies have shown that approximately 25% of all transcriptional units in the human and mouse genomes are involved in cis-sense-antisense pairs. However, the number of known sense-antisense pairs remains limited because currently available cDNA sequences represent only a fraction of the total number of transcripts comprising the transcriptome of each cell type. Results: To discover novel antisense transcripts encoded in the antisense strand of important genes, such as cancer-related genes, we conducted expression analyses of antisense transcripts using our custom microarray platform along with 2376 probes designed specifically to detect the potential antisense transcripts of 501 well-known genes suitable for cancer research. Using colon cancer tissue and normal tissue surrounding the cancer tissue obtained from 6 patients, we found that antisense transcripts without poly(A) tails are expressed from approximately 80% of these well-known genes. This observation is consistent with our previous finding that many antisense transcripts expressed in a cell are poly(A)-. We also identified 101 and 71 antisense probes displaying a high level of expression specifically in normal and cancer tissues respectively. Some of these probes showed characteristic expression patterns which are anti-correlated with the expression patterns of the sense genes. Conclusion: Our microarray analysis identified novel antisense transcripts with expression profiles specific to cancer tissue, some of which might play a role in the regulatory networks underlying oncogenesis and thus are potential targets for further experimental validation. 501 probes targeting well-known genes suitable for cancer researches were designed. Additionally 2,376 probes targeting putative transcription units on the antisense strand of these genes were designed. Samples were obtained from colon cancer tissues and normal tissues surrounding cancer tissues of six patients.

Dataset Information

Ave1 Homologs in Venturia pirina and Venturia inequalis

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