Proteomics

Dataset Information

229

Matrisome remodelling during Intervertebral Disc development and ageing


ABSTRACT: Intervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM remodelling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, was used to define the NP matrisome. The enrichment of Collagen XII and XIV in foetus, Fibronectin and Prolargin in elder samples and Collagen XI in young ones was independently validated. This study provides the first matrisome database of healthy discs during development and ageing, which is key to determine the pathways and processes that maintain disc homeostasis. The factors identified may help to explain age-associated IVD degeneration or constitute putative effectors for disc regeneration.

INSTRUMENT(S): 4700 Proteomics Analyzer

ORGANISM(S): Bos taurus  

TISSUE(S): Intervertebral Disc

DISEASE(S): Not Available

SUBMITTER: Hugo Osorio  

LAB HEAD: Hugo Osorio

PROVIDER: PXD005616 | Pride | 2017-09-25

REPOSITORIES: Pride

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Publications

Matrisome Profiling During Intervertebral Disc Development And Ageing.

Caldeira Joana J   Santa Cátia C   Osório Hugo H   Molinos Maria M   Manadas Bruno B   Gonçalves Raquel R   Barbosa Mário M  

Scientific reports 20170914 1


Intervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM profiling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, w  ...[more]

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