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Protein Expression in TW Formation

ABSTRACT: Tangential sections of 30 µm thickness were taken across the secondary phloem and developing secondary xylem, sections were then pooled to represent the tissues from specific developmental stages. Phloem (P) comprises 3 sections, the cambium (C) - 2 sections, the radial expansion zone (E) - 4 sections, the first to third xylem development zones (X1-X3) - 3 sections each, and the forth to sixth xylem development zones (X4-X6) - 6 sections each.

INSTRUMENT(S): Synapt G2 HDMS

ORGANISM(S): Populus Tremula

TISSUE(S): Wood

SUBMITTER: Joakim Bygdell

LAB HEAD: Gunnar Wingsle

PROVIDER: PXD005715 | Pride | 2017-06-23

REPOSITORIES: Pride

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Protein expression in tension wood formation monitored at high tissue resolution in Populus.

Bygdell Joakim J Srivastava Vaibhav V Obudulu Ogonna O Srivastava Manoj K MK Nilsson Robert R Sundberg Björn B Trygg Johan J Mellerowicz Ewa J EJ Wingsle Gunnar G

Journal of experimental botany 20170601 13

Tension wood (TW) is a specialized tissue with contractile properties that is formed by the vascular cambium in response to gravitational stimuli. We quantitatively analysed the proteomes of Populus tremula cambium and its xylem cell derivatives in stems forming normal wood (NW) and TW to reveal the mechanisms underlying TW formation. Phloem-, cambium-, and wood-forming tissues were sampled by tangential cryosectioning and pooled into nine independent samples. The proteomes of TW and NW samples ...[more]

PMID: 28633298

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