Dataset Information

ECM characterization in stented porcine coronary arteries LC-MS/MS

ABSTRACT: Bare-metal (BMS) and drug-eluting stents (DES) were implanted in pig coronary arteries with an overstretch during coronary angioplasty under optical coherence tomography guidance. Arteries subjected to plain old balloon angioplasty (POBA) alone served as controls. Stented/balloon dilated segments were harvested 1, 3, 7, 14 and 28 days post-intervention for proteomics analysis. At day 28 all stented arteries showed a neointima formation covering the stent struts. The evolved neointima was separated from the media and analysed in a separate proteomics analysis. In total, 31 samples were analysed for the media by LC-MS/MS (n=3 BMS/DES at each time-point 1, 3, 7 and 28 days; n=4 POBA early [day1-day3] and n=3 POBA late [day 14 - day28]). For the neointima a total of 14 samples were analysed (n=7 BMS, n=7 DES at 28 days) including the neointima of arteries of a second cohort with 4 samples each for BMS and DES day 28. The neointima samples were run in duplicates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Coronary Artery

SUBMITTER: Xiaoke Yin

LAB HEAD: Manuel Mayr

PROVIDER: PXD005726 | Pride | 2017-11-24

REPOSITORIES: Pride

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Publications

Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries.

Suna Gonca G Wojakowski Wojciech W Lynch Marc M Barallobre-Barreiro Javier J Yin Xiaoke X Mayr Ursula U Baig Ferheen F Lu Ruifang R Fava Marika M Hayward Robert R Molenaar Chris C White Stephen J SJ Roleder Tomasz T Milewski Krzysztof P KP Gasior Pawel P Buszman Piotr P PP Buszman Pawel P Jahangiri Marjan M Shanahan Catherine M CM Hill Jonathan J Mayr Manuel M

Circulation 20171013 2

<h4>Background</h4>Extracellular matrix (ECM) remodeling contributes to in-stent restenosis and thrombosis. Despite its important clinical implications, little is known about ECM changes post-stent implantation.<h4>Methods</h4>Bare-metal and drug-eluting stents were implanted in pig coronary arteries with an overstretch under optical coherence tomography guidance. Stented segments were harvested 1, 3, 7, 14, and 28 days post-stenting for proteomics analysis of the media and neointima.<h4>Results ...[more]

PMID: 29030347

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Dataset Information

ECM characterization in stented porcine coronary arteries LC-MS/MS

Publications

Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries.

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