Proteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations

ABSTRACT: The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with proteomics can be used to sort and analyze cell populations based on their metabolic state. A previously established GFP reporter system was used to detect and sort single Corynebacterium glutamicum cells based on the concentration of branched chain amino acids (BCAA) using FACS. A proteomics workflow optimized for small cell numbers was used to quantitatively compare proteomes of a ?aceE mutant, lacking functional pyruvate dehydrogenase (PD), and the wild type. About 800 proteins could be quantified from 1,000,000 cells. In the ?aceE mutant BCAA production was coordinated with upregulation of the glyoxylate cycle and TCA cycle to counter the lack of acetyl CoA resulting from the deletion of aceE.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Corynebacterium Glutamicum Atcc 13032

SUBMITTER: Andreas Harst  

LAB HEAD: PD. Dr. Ansgar Poetsch

PROVIDER: PXD005812 | Pride | 2017-11-02

REPOSITORIES: Pride