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SHOTGUN PROTEOMIC ANALYSIS TO STUDY THE DECREASE OF XENOGRAFT TUMOR GROWTH AFTER ROSEMARY EXTRACT TREATMENT

ABSTRACT: In the present work, the effects of rosemary extract on xenograft tumor growth has been studied and, for the first time, a shotgun proteomic analysis based on nanoliquid chromatography−tandem mass spectrometry (nano-LC−MS/MS) together with stable isotope dimethyl labeling (DML) has been applied to investigate the global protein changes in vivo.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colonic Cancer Cell Line, Epithelial Cell

DISEASE(S): Colon Adenocarcinoma

SUBMITTER: Alberto Valdés

LAB HEAD: Alejandro Cifuentes

PROVIDER: PXD005853 | Pride | 2017-04-10

REPOSITORIES: Pride

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Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment.

Valdés Alberto A García-Cañas Virginia V Pérez-Sánchez Almudena A Barrajón-Catalán Enrique E Ruiz-Torres Verónica V Artemenko Konstantin A KA Micol Vicente V Bergquist Jonas J Cifuentes Alejandro A

Journal of chromatography. A 20170327

The antiproliferative activity of Rosemary (Rosmarinus officinalis) has been widely studied in different in vitro and in vivo models, which demonstrate that rosemary extracts inhibit the cellular proliferation due to its ability to interact with a wide spectrum of molecular targets. However, a comprehensive proteomics study in vivo has not been carried out yet. In the present work, the effects of rosemary extract on xenograft tumor growth has been studied and, for the first time, a shotgun prote ...[more]

PMID: 28389096

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