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CARM1 substrate identification

ABSTRACT: The goal of the project was global identification of CARM1 substrates. Arginine methylation landscapes were profiled and compared in wild type and CARM1 knockout cells to determine in vivo substrates of CARM1.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Breast Epithelium, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Evgenia Shishkova

LAB HEAD: Joshua J Coon

PROVIDER: PXD005871 | Pride | 2017-07-03

REPOSITORIES: Pride

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Global mapping of CARM1 substrates defines enzyme specificity and substrate recognition.

Shishkova Evgenia E Zeng Hao H Liu Fabao F Kwiecien Nicholas W NW Hebert Alexander S AS Coon Joshua J JJ Xu Wei W

Nature communications 20170524

Protein arginine methyltransferases (PRMTs) introduce arginine methylation, a post-translational modification with the increasingly eminent role in normal physiology and disease. PRMT4 or coactivator-associated arginine methyltransferase 1 (CARM1) is a propitious target for cancer therapy; however, few CARM1 substrates are known, and its mechanism of substrate recognition is poorly understood. Here we employed a quantitative mass spectrometry approach to globally profile CARM1 substrates in brea ...[more]

PMID: 28537268

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Expression profiling of murine neonatal lung tissue from Carm1 knockouts and wild type controls

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