Shotgun Proteomic Analysis of Proteins Present in Mosquito Ovaries at 24 and 72 h After a Blood Meal

ABSTRACT: Female mosquitoes require a blood meal for oogenesis, and thereby receive a substantial iron load in the forms of holo-transferrin and hemoglobin. Our previous data showed that during digestion of a blood meal, the gut iron concentration decreases 10-fold, while that of the ovaries doubles from ingestion to 72 hours post feeding. Approximately 72 hours post feeding, eggs are laid with ~125 ng Fe each. We are interested in the effects of the blood meal on the expression of iron related proteins detected in the ovaries during time post feeding before eggs are laid. We have used shotgun proteomic analysis to identify proteins in the developing ovaries of Aedes aegypti; this information provides further insight into the effect of a blood meal on mosquito oogenesis.

INSTRUMENT(S):

ORGANISM(S): Aedes Aegypti

TISSUE(S): Ovary

SUBMITTER: D. L. Geiser  

LAB HEAD: Joy J. Winzerling

PROVIDER: PXD005893 | Pride | 2022-03-26

REPOSITORIES: Pride