Dataset Information

X!Tandem E. coli large vs small database

ABSTRACT: A comparison of the performance when using X!Tandem with a 1) large, exhaustive, reference database; and 2) small, specialized, database.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Fredrik Boulund

LAB HEAD: Edward R. B. Moore

PROVIDER: PXD005924 | Pride | 2017-04-21

REPOSITORIES: Pride

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Publications

Typing and Characterization of Bacteria Using Bottom-up Tandem Mass Spectrometry Proteomics.

Boulund Fredrik F Karlsson Roger R Gonzales-Siles Lucia L Johnning Anna A Karami Nahid N Al-Bayati Omar O Åhrén Christina C Moore Edward R B ERB Kristiansson Erik E

Molecular & cellular proteomics : MCP 20170418 6

Methods for rapid and reliable microbial identification are essential in modern healthcare. The ability to detect and correctly identify pathogenic species and their resistance phenotype is necessary for accurate diagnosis and efficient treatment of infectious diseases. Bottom-up tandem mass spectrometry (MS) proteomics enables rapid characterization of large parts of the expressed genes of microorganisms. However, the generated data are highly fragmented, making downstream analyses complex. Her ...[more]

PMID: 28420677

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Project description:Background: Alternative polyadenylation (APA) is emerging as a widespread mechanism of gene regulation. The usage of APA sites allows a single gene to encode multiple mRNA transcripts with different 3'-untranslated region (3'UTR) lengths. Many disease processes reflect the importance of the regulation of APA site switching. The objective of this study was to explore the profiling of tandem APA sites in nasal polyps compared with nasal uncinate process mucosa. Methods: Sequencing of APA sites (SAPAS) based on second-generation sequencing technology was undertaken to investigate the use of tandem APA sites and identify gene expression patterns in samples from the nasal polyps and nasal uncinate process mucosa of two patients with chronic rhinosinusitis with nasal polyps. The findings of the SAPAS analysis were validated via quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Results: First, the results showed a switching of 3'UTR lengths in nasal polyps compared with nasal uncinate process mucosa. From the two patients, 105 overlapping genes in the nasal polyps were switched to distal poly(A) sites, and 90 such genes were switched to proximal poly(A) sites. Several Gene Ontology terms were enriched in the list of genes with switched APA sites, including transcription regulation, cell cycle, apoptosis, and metabolism. Second, we detected genes that showed differential expression with at least a 3-fold difference between nasal polyp tissue and nasal uncinate process mucosa. Between the two sample types, 627 genes exhibited differential expression. The qRT-PCR results confirmed our SAPAS results. Conclusion: APA site-switching events of 3'UTRs are prevalent in nasal polyp tissue, and the regulation of gene expression mediated by APA may play an important role in the formation and persistence of nasal polyps. Our results may provide new insights into the possible pathophysiologic processes involved in nasal polyps. Investigate the use of tandem APA sites of 3'ends of mRNAs using second-generation sequencing technology.

Dataset Information

X!Tandem E. coli large vs small database

Publications

Typing and Characterization of Bacteria Using Bottom-up Tandem Mass Spectrometry Proteomics.

Similar Datasets

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