Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Blood Serum
DISEASE(S): Prostate Adenocarcinoma
SUBMITTER: Ravi Chand Bollineni
LAB HEAD: Bernd Thiede
PROVIDER: PXD005931 | Pride | 2018-02-08
REPOSITORIES: Pride
Bollineni Ravi Chand RC Koehler Christian Jeffrey CJ Gislefoss Randi Elin RE Anonsen Jan Haug JH Thiede Bernd B
Scientific reports 20180201 1
Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics. We present an approach for automated annotation of intact glycopeptide mass spectra. The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases. Automated annotation of both N- and O-linked glycopeptides was proven using stand ...[more]