Dataset Information

Mouse Hepatic Lipid Droplet Proteome under Fasting and Re-Feeding

ABSTRACT: One of the key functions of the mammalian liver is lipid metabolism. During fasting, lipid storage in the liver increases in order to reserve and provide energy for cellular functions. Upon re-feeding, this reserve of lipids is rapidly depleted; this change is visible, as the organelles responsible for lipid storage – lipid droplets (LDs) – drastically decrease in size following re-feeding. Little is known regarding LD proteome, or how it changes during the fasting/re-feeding transition. Our study investigated the hepatic LD proteome and how it changes between fasting and re-feeding conditions. For this purpose, LDs were isolated from 4 month-old C57BL/6 mice after a 24 hour fasting period, or a 24 hour fasting period followed by 6 hours of re-feeding. Proteins isolated from these LDs were subject to SDS-PAGE followed by in-gel trypsinization and LC-MS/MS. We identified a combined total of 941 proteins on hepatic LDs, of which 817 had quantifiable extracted ion chromatograms in at least 2 samples (n=6 total) and were not deemed contaminants. 777 of the 817 proteins were observed in both energetic states, with 33 being uniquely observed in fasted LDs, and 7 being uniquely observed in re-fed LDs.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hepatocyte, Liver

DISEASE(S): Disease Free

SUBMITTER: David Kramer

LAB HEAD: Richard Lehner

PROVIDER: PXD005977 | Pride | 2018-05-25

REPOSITORIES: Pride

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Publications

Fasting and refeeding induces changes in the mouse hepatic lipid droplet proteome.

Kramer David A DA Quiroga Ariel D AD Lian Jihong J Fahlman Richard P RP Lehner Richard R

Journal of proteomics 20180423

During fasting, the liver increases lipid storage as a mean to reserve and provide energy for vital cellular functions. After re-feeding, hepatocytes rapidly decrease the amount of triacylglycerol that is stored in lipid droplets (LDs), visible as the size of hepatic LDs significantly decreases after re-feeding. Little is known about the changes in the liver LD proteome that occur during the fasting/re-feeding transition. This study aimed to investigate the hepatic LD proteome in fasted and re-f ...[more]

PMID: 29698803

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Dataset Information

Mouse Hepatic Lipid Droplet Proteome under Fasting and Re-Feeding

Publications

Fasting and refeeding induces changes in the mouse hepatic lipid droplet proteome.

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