Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell
DISEASE(S): Prostate Adenocarcinoma
SUBMITTER: Wei Yang
LAB HEAD: Wei Yang
PROVIDER: PXD006001 | Pride | 2022-02-24
REPOSITORIES: Pride
Analytical chemistry 20190711 15
Protein <i>S</i>-acylation (also called palmitoylation) is a common post-translational modification whose deregulation plays a key role in the pathogenesis of many diseases. Acyl-biotinyl exchange (ABE), a widely used method for the enrichment of <i>S</i>-acylated proteins, has the potential of capturing the entire <i>S</i>-acylproteome in any type of biological sample. Here, we showed that current ABE methods suffer from a high background arising from the coisolation of non-<i>S</i>-acylated pr ...[more]