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On-line desalting of sodium dodecyl sulfate in mass spectrometry

ABSTRACT: We tested here the ability of weak cation exchange (WCX) stationary phase to retain positively charged proteinaceous whilst negatively charged SDS molecules can be washed out from the capillary during on-line desalting. This novel chromatographic method named as online desalting of sodium dodecyl sulfate (online deSDS) satisfactorily allows for the first time the unforeseen straightforward LC-MS/MS analysis of SDS-containing samples without the requirement of any additional pre-processing step.

INSTRUMENT(S): LTQ FT, LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: Aida Serra

LAB HEAD: Siu Kwan Sze

PROVIDER: PXD006035 | Pride | 2018-06-18

REPOSITORIES: Pride

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Online Removal of Sodium Dodecyl Sulfate via Weak Cation Exchange in Liquid Chromatography-Mass Spectrometry Based Proteomics.

Serra Aida A Gallart-Palau Xavier X Dutta Bamaprasad B Sze Siu Kwan SK

Journal of proteome research 20180620 7

Biological research often requires the use of sodium dodecyl sulfate (SDS) to solubilize protein samples; however, this detergent is not compatible with direct mass spectrometry (MS) analysis. Here, we report an online high-throughput proteomics method that permits standard in-solution digestion of SDS-containing samples followed by direct liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis using weak cation-exchange chromatography (WCX). This approach, called the onlin ...[more]

PMID: 29888605

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