On-line desalting of sodium dodecyl sulfate in mass spectrometry

ABSTRACT: We tested here the ability of weak cation exchange (WCX) stationary phase to retain positively charged proteinaceous whilst negatively charged SDS molecules can be washed out from the capillary during on-line desalting. This novel chromatographic method named as online desalting of sodium dodecyl sulfate (online deSDS) satisfactorily allows for the first time the unforeseen straightforward LC-MS/MS analysis of SDS-containing samples without the requirement of any additional pre-processing step.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: Aida Serra  

LAB HEAD: Siu Kwan Sze

PROVIDER: PXD006035 | Pride | 2018-06-18

REPOSITORIES: Pride