Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Toxoplasma Gondii
TISSUE(S): Foreskin Fibroblast
SUBMITTER: James Wright
LAB HEAD: Jyoti Choudhary
PROVIDER: PXD006045 | Pride | 2017-10-12
REPOSITORIES: Pride
Jia Yonggen Y Marq Jean-Baptiste JB Bisio Hugo H Jacot Damien D Mueller Christina C Yu Lu L Choudhary Jyoti J Brochet Mathieu M Soldati-Favre Dominique D
The EMBO journal 20171013 21
<i>Toxoplasma gondii</i> encodes three protein kinase A catalytic (PKAc1-3) and one regulatory (PKAr) subunits to integrate cAMP-dependent signals. Here, we show that inactive PKAc1 is maintained at the parasite pellicle by interacting with acylated PKAr. Either a conditional knockdown of PKAr or the overexpression of PKAc1 blocks parasite division. Conversely, down-regulation of PKAc1 or stabilisation of a dominant-negative PKAr isoform that does not bind cAMP triggers premature parasite egress ...[more]