Dataset Information

Comparative Proteomic Analysis of the Mycobacterium tuberculosis Lineage 7 Reveals Differentially Abundant Proteins Linked to Slow Growth

ABSTRACT: Differentially abundant proteins were quantified in the slowly growing Mycobacterium tuberculosis (M. tuberculosis) lineage 7 strains and the M. tuberculosis lineage 4 reference strain H37Rv. Mass spectrometry based proteomic analysis was employed to identify, quantitate and compare the protein profiles of strains from the two M. tuberculosis lineages (lineage 7 and 4). Label-free peptide quantification of whole cells from M. tuberculosis lineage 7 and 4 yielded the identification of 2825 and 2541 proteins, respectively. A combined total of 2867 protein groups covering 71% of the predicted M. tuberculosis proteome were identified. Comparative proteomic mapping of M. tuberculosis lineage 7 and 4 strains showed that the abundance of 125 proteins was significantly altered. Functional analysis showed that a number of M. tuberculosis proteins involved in growth and virulence were less abundant in lineage 7 compared to lineage 4. Five ABC transporter proteins, three phosphate binding proteins essential for inorganic phosphate uptake, and six components of the type 7 secretion system ESX-3 involved in iron acquisition were less abundant in M. tuberculosis lineage 7. This proteogenomic analysis provided an insight into the lineage 7-specific protein profile which may provide cues to understanding the differential properties of lineage 7 strains in terms of slow growth, survival fitness and pathogenesis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mycobacterium Tuberculosis H37rv

TISSUE(S): Cell Lysate

DISEASE(S): Tuberculosis

SUBMITTER: Shewit Kalayou

LAB HEAD: Prof Tone Tønjum

PROVIDER: PXD006117 | Pride | 2017-05-24

REPOSITORIES: Pride

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Publications

Comparative Proteomic Analysis of Mycobacterium tuberculosis Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence.

Yimer Solomon A SA Birhanu Alemayehu G AG Kalayou Shewit S Riaz Tahira T Zegeye Ephrem D ED Beyene Getachew T GT Holm-Hansen Carol C Norheim Gunnstein G Abebe Markos M Aseffa Abraham A Tønjum Tone T

Frontiers in microbiology 20170509

In order to decipher the nature of the slowly growing Mycobacterium tuberculosis (M.tuberculosis) lineage 7, the differentially abundant proteins in strains of M. tuberculosis lineage 7 and lineage 4 were defined. Comparative proteomic analysis by mass spectrometry was employed to identify, quantitate and compare the protein profiles of strains from the two M. tuberculosis lineages. Label-free peptide quantification of whole cells from M. tuberculosis lineage 7 and 4 ...[more]

PMID: 28536560

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Dataset Information

Comparative Proteomic Analysis of the Mycobacterium tuberculosis Lineage 7 Reveals Differentially Abundant Proteins Linked to Slow Growth

Publications

Comparative Proteomic Analysis of <i>Mycobacterium tuberculosis</i> Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence.

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