Proteomics

Dataset Information

0

Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan


ABSTRACT: Aging is associated with extensive remodeling of the heart, including basement membrane (BM) components that surround cardiomyocytes. Remodeling is thought to impair cardiac mechanotransduction, but the contribution of specific BM components to age-related lateral communication between cardiomyocytes is unclear. Using a genetically tractable, rapidly aging model with sufficient cardiac genetic homology and morphology, e.g. Drosophila melanogaster, we observed differential regulation of BM collagens between laboratory strains, correlating with changes in muscle physiology leading to cardiac dysfunction. Therefore, we sought to understand the extent to which BM proteins modulate contractile function during aging. Cardiac-restricted knockdown of ECM genes Pericardin, Laminin A, and Viking in Drosophila prevented age-associated heart tube restriction and increased contractility, even under viscous load. Most notably, reduction of Laminin A expression correlated with an overall preservation of contractile velocity with age and extension of organismal lifespan. Global heterozygous knockdown confirmed these data, which provides new evidence of a direct link between BM homeostasis, contractility, and maintenance of lifespan.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Heart

SUBMITTER: Koen Raedschelders  

LAB HEAD: Jennifer Van Eyk

PROVIDER: PXD006120 | Pride | 2017-03-27

REPOSITORIES: Pride

altmetric image

Publications

Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan.

Sessions Ayla O AO   Kaushik Gaurav G   Parker Sarah S   Raedschelders Koen K   Bodmer Rolf R   Van Eyk Jennifer E JE   Engler Adam J AJ  

Matrix biology : journal of the International Society for Matrix Biology 20161025


Aging is associated with extensive remodeling of the heart, including basement membrane (BM) components that surround cardiomyocytes. Remodeling is thought to impair cardiac mechanotransduction, but the contribution of specific BM components to age-related lateral communication between cardiomyocytes is unclear. Using a genetically tractable, rapidly aging model with sufficient cardiac genetic homology and morphology, e.g. Drosophila melanogaster, we observed differential regulation of BM collag  ...[more]

Similar Datasets

2022-09-26 | PXD033492 | Pride
2018-10-19 | PXD001842 | Pride
2016-04-15 | PXD001839 | Pride
2022-11-01 | GSE185967 | GEO
2022-11-01 | GSE185923 | GEO
2019-07-01 | GSE4728 | GEO
2020-04-01 | PXD018315 | Pride
2020-04-10 | GSE129793 | GEO
| E-GEOD-3181 | biostudies-arrayexpress
2023-11-03 | GSE218672 | GEO