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Phosphoproteome and proteome of T. cruzi metacyclogenesis

ABSTRACT: Trypanosoma cruzi metacyclogenesis is a natural process that occurs inside triatomine insect vector and corresponds to the differentiation of non-infective epimastigotes into infective metacyclic trypomastigotes. Here, centered on LC-MS/MS approach, a large scale phospho (proteome) study was carried out aiming to quantitatively understand the metacyclogenesis. Cells were colected along metacyclogenesis totalizing six points, stress 2 hours, adhered 12, 24 and 48 hours, and metacyclic trypomastigote from two biological replicates. After extraction, total protein were digested, fractioned and analyzed by LC-MS/MS. The samples for phosphoproteomics were processed for phosphopeptide enrichment prior to the analysis.

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Trypanosoma Cruzi Dm28c

SUBMITTER: juliana carolina amorim

LAB HEAD: Fabricio Klerynton Marchini

PROVIDER: PXD006171 | Pride | 2017-08-31

REPOSITORIES: Pride

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Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis.

Amorim Juliana C JC Batista Michel M da Cunha Elizabeth S ES Lucena Aline C R ACR Lima Carla V de Paula CVP Sousa Karla K Krieger Marco A MA Marchini Fabricio K FK

Scientific reports 20170829 1

Trypanosoma cruzi metacyclogenesis is a natural process that occurs inside the triatomine vector and corresponds to the differentiation of non-infective epimastigotes into infective metacyclic trypomastigotes. The biochemical alterations necessary for the differentiation process have been widely studied with a focus on adhesion and nutritional stress. Here, using a mass spectrometry approach, a large-scale phospho(proteome) study was performed with the aim of understanding the metacyclogenesis p ...[more]

PMID: 28852088

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Quantitative phosphoproteome and proteome analysis emphasize the influence of phosphorylation events during the nutritional stress of Trypanosoma cruzi: the initial moments of in vitro metacyclogenesis

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