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Bacillus cereus proteomics, intraproteome

ABSTRACT: This project contributes to the proteomic comparison of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid, its mutant strain devoided of the methionine sulfoxide reductase AB (msrAB) protein and the corresponding msrAB complemented strain, all strains grown in aerobiosis condition and harvested at three growth stages.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Bacillus Cereus (strain Atcc 14579 / Dsm 31)

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean ARMENGAUD

PROVIDER: PXD006205 | Pride | 2018-06-15

REPOSITORIES: Pride

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Time-course proteomics dataset to monitor protein-bound methionine oxidation in Bacillus cereus ATCC 14579.

Madeira Jean-Paul JP Alpha-Bazin Béatrice B Armengaud Jean J Duport Catherine C

Data in brief 20180310

Aerobic respiratory growth generates endogenous reactive oxygen species (ROS). ROS oxidize protein-bound methionine residues into methionine sulfoxide. Methionine sulfoxide reductases catalyze the reduction of methionine sulfoxide to methionine in proteins. Here, we use high-throughput nanoLC-MS/MS methodology to establish detailed maps of oxidized proteins from Bacillus cereus ATCC 14579 ΔpBClin15 and its mutant for which the methionine sulfoxide reductase AB gene (msrAB) has been ...[more]

PMID: 29896523

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