Dataset Information

Relative quantitative proteomics of uninfected vs wMel infected Aedes aegypti midguts

ABSTRACT: Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important new dengue control strategy. To better understand the mechanisms of virus inhibition, proteomic quantification of the effects of Wolbachia in mosquito (Aedes aegypti) midguts was performed.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Wolbachia Pipientis Wmelcs Aedes Aegypti

TISSUE(S): Midgut

SUBMITTER: Vincent Geoghegan

LAB HEAD: Steven P. Sinkins

PROVIDER: PXD006239 | Pride | 2017-09-19

REPOSITORIES: Pride

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Publications

Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells.

Geoghegan Vincent V Stainton Kirsty K Rainey Stephanie M SM Ant Thomas H TH Dowle Adam A AA Larson Tony T Hester Svenja S Charles Philip D PD Thomas Benjamin B Sinkins Steven P SP

Nature communications 20170913 1

Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control. To better understand the mechanisms of virus inhibition, we here perform proteomic quantification of the effects of Wolbachia in Aedes aegypti mosquito cells and midgut. Perturbations are observed in vesicular trafficking, lipid metabolism and in the endoplasmic reticulum that could impact viral entry a ...[more]

PMID: 28904344

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Project description:Background: Mosquito midgut is an important target for the host parasite interaction studies as this major immune organ plays a role in parasite growth, maturation and vector competence. Most important rural malaria vector of India is Proteomic approaches coupled with bioinformatics analysis have been used to study expression of functional proteins/enzymes of An. culicifacies susceptible and refractory species midgut in order to understand the mechanism of refractoriness that may help in contributing to unravel the host pathogen interactions Methodology/Principle findings: Basic shot gun proteomics approaches along with iTRAQ based differential studies followed by LC/MS/MS were employed to study the proteome of sugar fed An. culicifacies midgut. Further bioinformatics analysis was carried out using Gene ontology, SMART analysis, CELLO algorithm etc. Here proteins identified with iTRAQ Ratio more than 1.5 was considered up regulated and less than 0.67 were considered down regulated. Ratio from 1.5 – 0.67 were considered as moderate to no change. Using these criteria we observed total 80 proteins in An. culicifacies midgut and when compared susceptible species against refractory species, total 30 proteins were found to be downregulated. 3 proteins were found to be upregulated in refractory species Conclusion/Significance: Based on the present data a significant increase in number of proteins in midgut of refractory An. culicifacies species B were found that may conclude that these proteins may be responsible for the inhibiting parasite growth and linked to the melanization of oocysts or parasite lysis mechanisms in natural populations of refractory mosquito. Hence the progress of our studies at protein level suggests that these identified annotated putative proteins/enzymes may help to explore natural vector-parasite systems and reveal valuable insights into the mechanism of refractoriness which in turn further useful for bringing of novel strategies for control of malaria.

Dataset Information

Relative quantitative proteomics of uninfected vs wMel infected Aedes aegypti midguts

Publications

Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells.

Similar Datasets

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