Proteomics

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Global Landscape of Cell Envelope Protein Complexes in Escherichia coli

ABSTRACT: After extraction with mild non-denaturing detergents, we affinity-purified 785 endogenously-tagged CEPs and then identified stably-associated polypeptides by precision mass spectrometry. The resulting high-quality physical interaction network, comprising most (77%) of all targeted CEPs, revealed hundreds of previously unknown heteromeric complexes. Lab Heads: Andrew Emili; andrew.emili@utoronto.ca ;Donnelly CCBR, University of Toronto, Toronto ON M5S 3E1, Canada Mohan Babu; mohan.babu@uregina.ca ;Research and Innovation Centre, University of Regina, SK S4S OA2, Canada

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Escherichia Coli (strain K12 / W3110 / Atcc 27325 / Dsm 5911)

SUBMITTER: Sadhna Phanse  

LAB HEAD: Andrew Emili

PROVIDER: PXD006247 | Pride | 2017-11-30

REPOSITORIES: Pride

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Global landscape of cell envelope protein complexes in Escherichia coli.

Babu Mohan M   Bundalovic-Torma Cedoljub C   Calmettes Charles C   Phanse Sadhna S   Zhang Qingzhou Q   Jiang Yue Y   Minic Zoran Z   Kim Sunyoung S   Mehla Jitender J   Gagarinova Alla A   Rodionova Irina I   Kumar Ashwani A   Guo Hongbo H   Kagan Olga O   Pogoutse Oxana O   Aoki Hiroyuki H   Deineko Viktor V   Caufield J Harry JH   Holtzapple Erik E   Zhang Zhongge Z   Vastermark Ake A   Pandya Yogee Y   Lai Christine Chieh-Lin CC   El Bakkouri Majida M   Hooda Yogesh Y   Shah Megha M   Burnside Dan D   Hooshyar Mohsen M   Vlasblom James J   Rajagopala Sessandra V SV   Golshani Ashkan A   Wuchty Stefan S   F Greenblatt Jack J   Saier Milton M   Uetz Peter P   F Moraes Trevor T   Parkinson John J   Emili Andrew A  

Nature biotechnology 20171127 1

Bacterial cell envelope protein (CEP) complexes mediate a range of processes, including membrane assembly, antibiotic resistance and metabolic coordination. However, only limited characterization of relevant macromolecules has been reported to date. Here we present a proteomic survey of 1,347 CEPs encompassing 90% inner- and outer-membrane and periplasmic proteins of Escherichia coli. After extraction with non-denaturing detergents, we affinity-purified 785 endogenously tagged CEPs and identifie  ...[more]

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