Global Landscape of Cell Envelope Protein Complexes in Escherichia coli

ABSTRACT: After extraction with mild non-denaturing detergents, we affinity-purified 785 endogenously-tagged CEPs and then identified stably-associated polypeptides by precision mass spectrometry. The resulting high-quality physical interaction network, comprising most (77%) of all targeted CEPs, revealed hundreds of previously unknown heteromeric complexes. Lab Heads: Andrew Emili; [e-mail] ;Donnelly CCBR, University of Toronto, Toronto ON M5S 3E1, Canada Mohan Babu; [e-mail] ;Research and Innovation Centre, University of Regina, SK S4S OA2, Canada

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli (strain K12 / W3110 / Atcc 27325 / Dsm 5911)

SUBMITTER: Sadhna Phanse  

LAB HEAD: Andrew Emili

PROVIDER: PXD006247 | Pride | 2017-11-30

REPOSITORIES: Pride