Proteomics

Dataset Information

68

Proteome Analysis of Human Embryonic Stem Cells Organelles


ABSTRACT: Here, we have utilized a sub-cellular proteomics approach to analyze the localization of proteins in the nucleus, mitochondria, crude membrane, cytoplasm, heavy and light microsomes. Out of 2002 reproducibly identified proteins, we detected 762 proteins in a single organelle whereas 160 proteins were found in all sub-cellular fractions. We verified the localization of identified proteins through databases and discussed the consistency of the obtained results. With regards to the ambiguity in the definition of a membrane protein, we tried to clearly define the plasma membrane, peripheral membrane and membrane proteins by annotation of these proteins in databases, along with predictions of transmembrane helices.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo sapiens  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Faezeh Shekari  

LAB HEAD: Ghasem Hosseini Salekdeh

PROVIDER: PXD006271 | Pride | 2017-04-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Crude membrane (rep1).mgf Mgf
Crude membrane (rep1).mzml Mzml
Crude membrane (rep1).xml Xml
Crude membrane (rep2).mgf Mgf
Crude membrane (rep2).mzml Mzml
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Publications

Proteome analysis of human embryonic stem cells organelles.

Shekari Faezeh F   Nezari Hossein H   Larijani Mehran Rezaei MR   Han Chia-Li CL   Baharvand Hossein H   Chen Yu-Ju YJ   Salekdeh Ghasem Hosseini GH  

Journal of proteomics 20170420


As the functions of proteins are associated with their cellular localization, the comprehensive sub-cellular proteome knowledge of human embryonic stem cells (hESCs) is indispensable for ensuring a therapeutic effect. Here, we have utilized a sub-cellular proteomics approach to analyze the localization of proteins in the nucleus, mitochondria, crude membrane, cytoplasm, heavy and light microsomes. Out of 2002 reproducibly identified proteins, we detected 762 proteins in a single organelle wherea  ...[more]

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