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Membrane Proteome Profiling of Peripheral Immune Cells in Non-Small Cell Lung Cancer Patients Treated with First Line Combination Chemotherapy

ABSTRACT: Four lung adenocarcinoma patients with progression free survival (PFS) ranged from 2.13 to 14.5 months were recruited in the study. Paired PBMCs were collected before and after first regimen of PEM/CIS treatment for individualized analysis of membrane proteome profiles. The membrane protein fractions were purified from PBMCs, subjected to gel-assisted digestion, labeled with iTRAQ reagents, fractionated by high-pH reversed phase StageTip, and analyzed in duplicate by LC-MS/MS. Protein identification and quantitation was obtained by using Mascot with 1% false discovery rate and Multi-Q.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Peripheral Blood Mononuclear Cell, Blood

DISEASE(S): Lung Adenocarcinoma

SUBMITTER: Denise Utami Putri

LAB HEAD: Chia-Li Han

PROVIDER: PXD006297 | Pride | 2018-01-02

REPOSITORIES: Pride

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Chemotherapy Immunophenoprofiles in Non-Small-Cell Lung Cancer by Personalized Membrane Proteomics.

Putri Denise Utami DU Feng Po-Hao PH Hsu Yuu-Hueih YH Lee Kang-Yun KY Jiang Feng-Wen FW Kuo Lu-Wei LW Chen Yu-Ju YJ Han Chia-Li CL

Proteomics. Clinical applications 20180119 2

<h4>Objectives</h4>No study has addressed how the immune status at the molecular level is affected by first-line pemetrexed and cisplatin (PEM-CIS) combination therapy in patients with non-small-cell lung cancer (NSCLC). Thus, we aimed to identify the immune status from membrane proteome alterations in patients with NSCLC upon PEM-CIS treatment.<h4>Methods</h4>The paired peripheral blood mononuclear cells (PBMCs) were collected from four patients with lung adenocarcinoma before and after the fir ...[more]

PMID: 29278294

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