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Bod1 interactome in mitotic HeLa cells

ABSTRACT: To discover candidate proteins that can target Bod1 to kinetochores in mitotic HeLa cells, we combined affinity purification of Bod1 with label-free quantitative mass spectrometry.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Hela Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Katharina Schleicher

LAB HEAD: Jason R Swedlow

PROVIDER: PXD006322 | Pride | 2018-10-24

REPOSITORIES: Pride

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The Ndc80 complex targets Bod1 to human mitotic kinetochores.

Schleicher Katharina K Porter Michael M Ten Have Sara S Sundaramoorthy Ramasubramanian R Porter Iain M IM Swedlow Jason R JR

Open biology 20171101 11

Regulation of protein phosphatase activity by endogenous protein inhibitors is an important mechanism to control protein phosphorylation in cells. We recently identified Biorientation defective 1 (Bod1) as a small protein inhibitor of protein phosphatase 2A containing the B56 regulatory subunit (PP2A-B56). This phosphatase controls the amount of phosphorylation of several kinetochore proteins and thus the establishment of load-bearing chromosome-spindle attachments in time for accurate separatio ...[more]

PMID: 29142109

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