Dataset Information

A Parts List for Fungal Cellulosomes Revealed by Comparative Genomics

ABSTRACT: Characterization of proteins critical to fungal cellulosome assembly for Anaeromyces robustus, Neocallimastix californiae and Piromyces finnis (cellulosomes are multi-protein complexes that tether plant biomass degrading enzymes together). These findings suggest that the fungal cellulosome is an independently evolved fungal complex that co-opted useful activities from bacterial neighbors within the herbivore rumen microbiome.

INSTRUMENT(S):

ORGANISM(S): Neocallimastix Sp. G-1 Piromyces Sp. Anaeromyces

SUBMITTER: Matthew Monroe

LAB HEAD: Michelle O'Malley

PROVIDER: PXD006325 | Pride | 2018-10-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AOM_S4_01_9May14_Lynx_13-11-15.mgf	Mgf
	AOM_S4_01_9May14_Lynx_13-11-15.raw	Raw
	AOM_S4_01_9May14_Lynx_13-11-15_msgfplus.mzid.gz	Mzid
	AOM_S4_01_R2_9May14_Lynx_13-11-16.mgf	Mgf
	AOM_S4_01_R2_9May14_Lynx_13-11-16.raw	Raw

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Publications

A parts list for fungal cellulosomes revealed by comparative genomics.

Haitjema Charles H CH Gilmore Sean P SP Henske John K JK Solomon Kevin V KV de Groot Randall R Kuo Alan A Mondo Stephen J SJ Salamov Asaf A AA LaButti Kurt K Zhao Zhiying Z Chiniquy Jennifer J Barry Kerrie K Brewer Heather M HM Purvine Samuel O SO Wright Aaron T AT Hainaut Matthieu M Boxma Brigitte B van Alen Theo T Hackstein Johannes H P JHP Henrissat Bernard B Baker Scott E SE Grigoriev Igor V IV O'Malley Michelle A MA

Nature microbiology 20170530

Cellulosomes are large, multiprotein complexes that tether plant biomass-degrading enzymes together for improved hydrolysis1. These complexes were first described in anaerobic bacteria, where species-specific dockerin domains mediate the assembly of enzymes onto cohesin motifs interspersed within protein scaffolds1. The versatile protein assembly mechanism conferred by the bacterial cohesin-dockerin interaction is now a standard design principle for synthetic biology2, ...[more]

PMID: 28555641

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