Dataset Information

Determination of the specificity profiling of native and synthetic cardosin B using proteomic identification of protease cleavage sites (PICS)

ABSTRACT: We performed an analysis of the substrate specificity preferences of native and synthetic cardosin B using the Proteomic Identification of protease Cleavage Sites methodology and some differences regarding preference and stringency of the subsite binding pockets were observed. Results clearly suggest that synthetic cardosin B is more stringent than native cardosin B explaining the observed suitability of the synthetic protein as biotechnological tool in cheese industry.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Blood Cell, Monocyte, Blood

SUBMITTER: Rui Cruz

LAB HEAD: Isaura Simões

PROVIDER: PXD006329 | Pride | 2018-05-04

REPOSITORIES: Pride

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Publications

Functional and structural characterization of synthetic cardosin B-derived rennet.

Almeida Carla Malaquias CM Manso José A JA Figueiredo Ana C AC Antunes Liliana L Cruz Rui R Manadas Bruno B Bur Daniel D Pereira Pedro José Barbosa PJB Faro Carlos C Simões Isaura I

Applied microbiology and biotechnology 20170802 18

The potential of using a synthetic cardosin-based rennet in cheese manufacturing was recently demonstrated with the development and optimization of production of a recombinant form of cardosin B in Kluyveromyces lactis. With the goal of providing a more detailed characterization of this rennet, we herein evaluate the impact of the plant-specific insert (PSI) on cardosin B secretion in this yeast, and provide a thorough analysis of the specificity requirements as well as the biochemical and struc ...[more]

PMID: 28770303

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Dataset Information

Determination of the specificity profiling of native and synthetic cardosin B using proteomic identification of protease cleavage sites (PICS)

Publications

Functional and structural characterization of synthetic cardosin B-derived rennet.

Similar Datasets

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