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Comparative Evaluation of Five Protocols for Protein Extraction from Stony Corals (Scleractinia) for Proteomics

ABSTRACT: Corals especially the reef-building species are very important to marine ecosystems. Proteomics has been used for researches on coral diseases, bleaching and responses to the environment change. Corals especially the reef-building species are very important to marine ecosystems. Proteomics has been used for researches on coral diseases, bleaching and responses to the environment change. In the present study, five protocols were compared for protein extraction from stony corals.

INSTRUMENT(S): TOF/TOF 5800

ORGANISM(S): Acropora Digitifera Nematostella Vectensis Chromera Velia Exaiptasia Pallida

TISSUE(S): Whole Body

SUBMITTER: Huamin Cheng

LAB HEAD: Hongwei Zhao; Xiaoping Diao

PROVIDER: PXD006334 | Pride | 2022-07-24

REPOSITORIES: Pride

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Comparative evaluation of five protocols for protein extraction from stony corals (Scleractinia) for proteomics.

Cheng Huamin H Zhao Hongwei H Yang Tinghan T Ruan Sunlan S Wang Haihua H Xiang Nan N Zhou Hailong H Li Qing X QX Diao Xiaoping X

Electrophoresis 20180212 8

Corals especially the reef-building species are very important to marine ecosystems. Proteomics has been used for researches on coral diseases, bleaching and responses to the environment change. A robust and versatile protein extraction protocol is required for coral proteomics. However, a comparative evaluation of different protein extraction protocols is still not available for proteomic analysis of stony corals. In the present study, five protocols were compared for protein extraction from st ...[more]

PMID: 29363786

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