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Metaproteomics of phototrophic biomats from two soda lakes in the Canadian Rocky Mountains

ABSTRACT: This data is a case study done in the context of developing methods for assessing the taxonomic composition of microbial communities using metaproteomics. For this study with analyzed phototrophic biomats from two Soda Lakes in the Canadian Rocky Mountains using metaproteomics. For protein identification we generated a metagenome from which we predicted and annotated the protein sequences used to analyze the metaproteomes. The database is available in this PRIDE submission. Lake1 refers to Goodenough Lake (GEM, 51°19'47.64"N 121°38'28.90"W) and Lake2 referes to Last Chance Lake (LCM, 51°19'39.3" N 121°37'59.3"W).

OTHER RELATED OMICS DATASETS IN: PRJEB19901

INSTRUMENT(S): Q Exactive

ORGANISM(S): Archaea Bacteria Viruses Eukaryota (eucaryotes)

SUBMITTER: Manuel Kleiner

LAB HEAD: Marc Strous

PROVIDER: PXD006343 | Pride | 2017-05-12

REPOSITORIES: Pride

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Assessing species biomass contributions in microbial communities via metaproteomics.

Kleiner Manuel M Thorson Erin E Sharp Christine E CE Dong Xiaoli X Liu Dan D Li Carmen C Strous Marc M

Nature communications 20171116 1

Microbial community structure can be analyzed by quantifying cell numbers or by quantifying biomass for individual populations. Methods for quantifying cell numbers are already available (e.g., fluorescence in situ hybridization, 16-S rRNA gene amplicon sequencing), yet high-throughput methods for assessing community structure in terms of biomass are lacking. Here we present metaproteomics-based methods for assessing microbial community structure using protein abundance as a measure for biomass ...[more]

PMID: 29146960

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