Proteomics

Dataset Information

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Label-free quantitative proteomic analysis of Brucella abortus and Brucella melitensis


ABSTRACT: Brucella type strains (B. abortus 544 and B. melitensis 16 M), field-isolates (B. abortus T and B. melitensis C) were independently cultivated six times on Tryptic Soy Broth at 37 °C in the presence of 5% CO2 until mid-logarithmic phase. The cells were harvested by brief centrifugation and the bacteria was inactivated using recommended ethanol precipitation procedure. Following which the cells were lysed, proteins quantified and the differentially expressed proteins among B. abortus and B. melitensis were identified using liquid chromatography-mass spectrometry (LC/MS) based label free quantitative proteomics analysis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Brucella Abortus Brucella Melitensis

DISEASE(S): Brucella Melitensis Brucellosis,Brucellosis,Brucella Abortus Brucellosis

SUBMITTER: Jayaseelan Murugaiyan  

LAB HEAD: Jayaseelan Murugaiyan

PROVIDER: PXD006348 | Pride | 2020-06-11

REPOSITORIES: Pride

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Publications

Pan-Proteomic Analysis and Elucidation of Protein Abundance among the Closely Related <i>Brucella</i> Species, <i>Brucella abortus</i> and <i>Brucella melitensis</i>.

Murugaiyan Jayaseelan J   Eravci Murat M   Weise Christoph C   Roesler Uwe U   Sprague Lisa D LD   Neubauer Heinrich H   Wareth Gamal G  

Biomolecules 20200530 6


Brucellosis is a zoonotic infection caused by bacteria of the genus <i>Brucella</i>. The species, <i>B. abortus</i> and <i>B. melitensis</i>, major causative agents of human brucellosis, share remarkably similar genomes, but they differ in their natural hosts, phenotype, antigenic, immunogenic, proteomic and metabolomic properties. In the present study, label-free quantitative proteomic analysis was applied to investigate protein expression level differences. Type strains and field strains were  ...[more]

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