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Identification of Siglec ligands in human B cell

ABSTRACT: Siglecs are a family of receptor-type glycan recognition proteins (lectins) involved in self - nonself discrimination by the immune system. Identification of Siglec ligand(s) is necessary to understand how Siglec-ligand interaction translates to biological outcomes, but is challenging because the interaction is weak. To facilitate identification of Siglec ligands, we adopted proximity labeling method based on tyramide radicalization principle.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): B Cell

DISEASE(S): Lymphoma

SUBMITTER: Yi-Ju Chen

LAB HEAD: Takashi Angata

PROVIDER: PXD006359 | Pride | 2017-09-29

REPOSITORIES: Pride

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Identification of Siglec Ligands Using a Proximity Labeling Method.

Chang Lanyi L Chen Yi-Ju YJ Fan Chan-Yo CY Tang Chin-Ju CJ Chen Yi-Hsiu YH Low Penk-Yeir PY Ventura Albert A Lin Chun-Cheng CC Chen Yu-Ju YJ Angata Takashi T

Journal of proteome research 20170922 10

Siglecs are a family of receptor-type glycan recognition proteins (lectins) involved in self-nonself discrimination by the immune system. Identification of Siglec ligands is necessary to understand how Siglec-ligand interaction translates into biological outcomes. However, this is challenging because the interaction is weak. To facilitate identification of Siglec ligands, we adopted a proximity labeling method based on the tyramide radicalization principle. Cells that express Siglec ligands were ...[more]

PMID: 28899088

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