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Mus_Oocytes _LC-MS/MS - Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice

ABSTRACT: A quantitative mass-spectrometry-based proteome profiles of oocytes

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Oocyte

SUBMITTER: hao zhang

LAB HEAD: Xuejiang Guo

PROVIDER: PXD006408 | Pride | 2018-10-24

REPOSITORIES: Pride

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			Action	DRS
	Mouse-egg-TMT6-HP-RP-1-20160130.raw	Raw
	Mouse-egg-TMT6-HP-RP-10-20160130.raw	Raw
	Mouse-egg-TMT6-HP-RP-11-20160130.raw	Raw
	Mouse-egg-TMT6-HP-RP-12-20160130.raw	Raw
	Mouse-egg-TMT6-HP-RP-13-20160130.raw	Raw

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Publications

Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

Guo Jing J Zhang Teng T Guo Yueshuai Y Sun Tao T Li Hui H Zhang Xiaoyun X Yin Hong H Cao Guangyi G Yin Yaoxue Y Wang Hao H Shi Lanying L Guo Xuejiang X Sha Jiahao J Eppig John J JJ Su You-Qiang YQ

Proceedings of the National Academy of Sciences of the United States of America 20180521 23

MTOR (mechanistic target of rapamycin) is a widely recognized integrator of signals and pathways key for cellular metabolism, proliferation, and differentiation. Here we show that conditional knockout (cKO) of <i>Mtor</i> in either primordial or growing oocytes caused infertility but differentially affected oocyte quality, granulosa cell fate, and follicular development. cKO of <i>Mtor</i> in nongrowing primordial oocytes caused defective follicular development leading to progressive degeneratio ...[more]

PMID: 29784807

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Mus_Oocyte_LC-MS/MS-mRBPome capture of oocytes identifies the RNA-binding protein LSM14B, an essential regulator of meiosis in mouse oocytes

Project description:A quantitative mass-spectrometry-based proteome profiles of oocytes in WT and Lsm14b KO mouse

2023-07-20 | PXD034070 | Pride

Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

Project description: Not available

| S-EPMC6003357 | biostudies-literature

Preconception diet interventions in obese outbred mice and the impact on female offspring metabolic health and oocyte quality

Project description:Sensitivity of murine offspring’s oocytes to a maternal high-fat/high-sugar (HF/HS) diet and how preconception care interventions (diet normalization (DN) or caloric restriction (CR)) might influence this. A proteomic insight.

2024-02-15 | PXD049281 | Pride

TMT-MS Mouse oocyte proteomics dataset

Project description:This dataset contains quantitative tandem mass tag (TMT)-based proteomic profiling of mouse oocytes across defined experimental conditions. 500, fully grown, prophase I-arrested oocytes were isolated from young adult females and matured in vitro to metaphase II eggs.

2026-06-06 | PXD076290 | Pride

Protein profiling of mouse fully-grown oocyte

Project description:To verify the Ribo-seq data of mouse oocyte, we performed MS/MS on mouse fully-grown oocytes. And the results show that our Ribo-seq data well reflect the proteomic dynamics in the fully grown oocytes.

2022-03-14 | PXD032229 | Pride

Human oocyte proteome and secretome

2016-06-01 | PXD004142 | Pride

Human oocyte proteome and secretome

Project description:The proteome and secretome of human oocytes was studied by shotgun LCMSMS, starting from pools of 100 cells. Exploiting SP3, a novel technology for proteomic sample preparation using magnetic beads, we scaled down proteome analysis to single cells. Despite the low protein content of only ~100ng per cell, we consistently identified ~450 proteins from individual oocytes. When comparing proteomes of individual oocytes at the germinal vesicle (GV) and metaphase II (MII) stage by label-free quantification, we found several proteins preferentially expressed in matuare and immature cells. This study demonstrates that an innovative proteomics workflow facilitates analysis of single human oocytes to investigate human oocyte biology and pre-implantation development. The approach presented here paves the way for quantitative proteomics in other quantity-limited tissues and cell types.

2016-05-26 | PXD003691 | Pride

Effects of in vitro maturation on oocyte gene expression

Project description:In vitro oocyte maturation (IVM) holds great promise as a tool for enhancing clinical treatment of infertility, enhancing availability of non human primates for development of disease models, and facilitating endangered species preservation. However, IVM outcomes have remained significantly below success rates obtained using in vivo matured (VVM) oocytes from humans and non human primates. A cDNA array based analysis is presented, comparing the transcriptomes of VVM oocytes with IVM oocytes. We observe a small set of just 59 mRNAs that are differentially expressed between the two cell types. These mRNAs are related to cellular homeostasis, cell-cell interactions including growth factor and hormone stimulation and cell adhesion, and other functions such as mRNA stability and translation. Additionally, we observe in IVM oocytes overexpression of PLAGL1 and MEST, two maternally imprinted genes, indicating a possible interruption or loss of correct epigenetic programming. These results indicate that, under certain IVM conditions, oocytes that are molecularly highly similar to VVM oocytes can be obtained, however the interruption of normal oocyte-somatic cell interactions during the final hours of oocyte maturation may preclude the establishment of full developmental competence. Keywords: oocyte maturation Comparison of in vitro matured MII-stage oocytes (4 biological replicates) with in vivo matured MII-stage oocytes (4 biological replicates)

2009-05-20 | E-GEOD-11895 | biostudies-arrayexpress

Proteomic-based identification of oocyte maturation-related proteins in mouse GV oocytes

Project description:In this study, we applied 1D SDS-PAGE and RP-LC-MS/MS to investigate the proteins stored in GV mouse oocytes. This high-performance strategy allowed us to define a set of 1405 different mouse GV oocyte proteins. It is confirmed that this study will help us to understand the diverse biological processes occurring in mouse oocytes and during early embryo development. However, compared with proteomic analysis of other cells and tissues, such as embryonic stems from cells and liver, the proteins identified in mature mouse oocytes were limited. This was mainly due to the fact that oocytes obtained from each mouse were very limited. We believe that the catalog of maternal proteins presented in this article is a starting point and we anticipate that more researches on the oocyte proteome will deduce most of the maternal proteins.

2020-09-17 | PXD014314 | Pride

Evaluation of ribonucleic acid amplification protocols for human oocyte transcriptome analysis

Project description:Objective: To develop a reliable, reproducible, and sensitive method for investigating gene-expression profiles from individual human oocytes. Setting: University medical center and university microarray laboratory. Patient(s): Couples undergoing intracytoplasmic sperm injection treatment were asked to donate their immature oocytes for research, and written informed consent was obtained in all cases. Seventy-nine human oocytes were used in total. Intervention(s): None. Main Outcome Measure(s): Amplification efficiency and microarray profiles. Result(s): Two of the five protocols (WT-Ovation One-Direct and Arcturus RiboAMp HS Plus) resulted in sufficient yields and high success rates and were further validated for their performance in obtaining reliable, reproducible, and sensitive expression profiles from individual human oocytes. Evaluation of these two protocols demonstrated that they both displayed low technical variation and produced highly reproducible profiles (r R 0.95). One of them identified significantly more transcripts but also had a higher number of false discoveries. Conclusion(s): Two protocols generated ample amounts of mRNA for (quantitative) polymerase chain reaction, microarray, and sequencing techniques. Further validation using a design that discriminates between biological and technical variation showed that both protocols can be used for gene-expression profiling of individual human oocytes. Design: Five commercially available protocols were investigated for their efficiency to amplify messenger RNA (mRNA) from 54 single human oocytes. Protocols resulting in sufficient yields were further validated using microarray technology. For the validation, mRNA was isolated from 25 human oocytes. To eliminate biological variation, RNA from 13 human oocytes was pooled together and split into 12 identical samples for further mRNA amplification. From 12 oocytes, mRNA was individually isolated.

2015-12-31 | E-GEOD-48141 | biostudies-arrayexpress

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