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The effect of Fusarium culmorum infection and deoxynivalenol (DON) application on proteome response in barley cultivars Chevron and Pedant

ABSTRACT: The effect of an artificial infection with Fusarium culmorum and an application of deoxynivalenol (DON) on barley spikes of cultivars Chevron and Pedant during flowering was investigated at grain mid-dough stage 10 days after pathogen inoculation (10 dai). Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with LC-MS/MS was used to investigate the quantitative or qualitative differences between the experimental variants.

INSTRUMENT(S): maXis

ORGANISM(S): Hordeum Vulgare (barley) Fusarium Culmorum

TISSUE(S): Spike

SUBMITTER: Jiri Santrucek

LAB HEAD: Pavel Vitamvas

PROVIDER: PXD006432 | Pride | 2017-09-07

REPOSITORIES: Pride

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The effect of Fusarium culmorum infection and deoxynivalenol (DON) application on proteome response in barley cultivars Chevron and Pedant.

Kosová Klára K Chrpová Jana J Šantrůček Jiří J Hynek Radovan R Štěrbová Lenka L Vítámvás Pavel P Bradová Jana J Prášil Ilja Tom IT

Journal of proteomics 20170713

Fusarium head blight (FHB) disease adversely affects grain quality and final yield in small-grain cereals including barley. In the present study, the effect of an artificial infection with Fusarium culmorum and an application of deoxynivalenol (DON) on barley spikes of cultivars Chevron and Pedant during flowering was investigated at grain mid-dough stage (BBCH 73) 10days after pathogen inoculation (10 dai). Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) te ...[more]

PMID: 28713028

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